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24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018


Laboratory of Plant-Microbe Interactions - LIPM

Laboratory of Plant-Microbe Interactions

Endosymbiotic infection and nodule development

Endosymbiotic associations between plants and soil microorganisms play key roles in improving the supply of essential nutrients to the host plant. We work on the nitrogen-fixing symbiotic interaction between legume plants and soil bacteria collectively known as rhizobia, which is of particular interest to reduce synthetic nitrogen fertilizer inputs in agriculture. This sophisticated interaction results in the development of a new root organ, called the root nodule, within which rhizobia are hosted to convert atmospheric nitrogen into forms the plant can use. The formation of this remarkable organ requires bacterial colonization and nodule development to occur in a tightly synchronized manner. Our team uses the model legume Medicago truncatula to investigate the plant regulatory mechanisms that govern rhizobial infection, nodule development and their coordination. Our main scientific objectives are to understand (i) how the plant controls infection-related cell-type specific responses and (ii) how transcriptional and epigenetic regulatory mechanisms shape nodule development. Our projects thus employ a range of approaches, from classical ones (molecular biology, genetics, transcriptomics, (epi)genomics and microscopy) to more advanced, cutting edge methods (in cell biology, reverse genetics and cell type-specific approaches) that we have recently adapted for Medicago. A general outline of our current projects in infection and nodule developmental processes is described in the next “Research Themes section”.