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INRA
24, chemin de Borde Rouge –Auzeville – CS52627
31326 Castanet Tolosan CEDEX - France

Dernière mise à jour : Mai 2018

Menu LIPM logo INRAE CNRS

Laboratory of Plant-Microbe Interactions - LIPM

Laboratory of Plant-Microbe Interactions

Non Legumes - Benoit Lefebvre

Symbiotic signal perception and arbuscular mycorrhiza in non-legumes.

The arbuscular mycorrhizal (AM) symbiosis. Most plants including cereals form an endosymbiosis with AM fungi belonging to the group of Glomeromycota. AM fungi are beneficial for plants because of their ability to colonize both soil and roots and collect nutrients from a much higher soil volume than plant roots. AM fungi penetrate root cortical cells and exchange nutrients through structures called arbuscules. AM fungi provide soil nutrients (phosphorus, nitrogen and other nutrients) to plants and benefit from fixed carbon (photosynthates) from the plants through carbohydrate and lipid tranfers. AM fungi can also protect plants from biotic and abiotic stress. This interaction is described as a mutualistic symbiosis, however antagonistic effects on plant host growth can sometimes be observed.

tomato root

A tomato root colonized by a strain of the AM fungus Rhizophagus irregularis. The fungus is stained by ink (blue). Arrows show arbuscules. Arrowheads show extraradical hyphae.

Our research: We are interested in understanding plant molecular mechanisms which influence 1/ host colonization by AM fungi and 2/ mycorrhizal growth response (which can positive or negative).

B. distachyon

Plant models: In 2012, I initiated studies on model and crop plants including dicots: Nicotiana benthamiana and Solanum lycopersicum (tomato) and monocots: Brachypodium distachyon and Triticum aestivum (wheat).

A B. distachyon plant grown for 9 weeks in a growth chamber.

Note that Arabidopsis thaliana, a widely used plant model is one of the few plants unable to form the AM symbiosis.

Binding assays

Objective 1: To determine the role of signal molecules produced by AM fungi in the establishment of the symbiosis. Lipo-chitooligosaccharidic (LCO) and short chitooligosaccharidic (CO) produced by AM fungi are able to activate a host signalling pathway required for host colonization by AM fungi and thus LCOs and COs are likely involved in mechanisms of host colonization. Our strategy consists of the identification and characterization of plant LCO and CO receptors. Candidates belong to the family of Receptor-Like Kinases (RLKs) containing LysM domains. We are characterizing LCO or CO binding properties of LysM-RLKs using radiolabelled molecules or Microscale Thermophoresis. We are also determining the biological roles of these receptors by reverse genetic approaches. We also aim to purify LCO or CO binding proteins from membrane fractions and to identify them by mass spectrometry.

plant toulouse phenotyping platform

Objective 2: To identity the genetic determinants and the molecular mechanisms that control plant benefit from AM fungi. To address this objective, we are investigating the natural genetic variability of B. distachyon. This includes analysis of growth and transcriptional responses of plants to various AM fungal strains in various environments. The aim is to identify molecular mechanisms that are critical for mycorrhizal growth responses using quantitative genetic approaches. For this, we use the new Toulouse plant phenotyping platform to measure growth kinetics of various B. distachyon genotypes in the presence or the absence of AM fungi.

wheat root epiphytes

Objective 3: Characterize functional interactions between AM fungi and soil bacteria and measure effect of agricultural practices on AM fungal and associated bacterial communities. We have initiated a research program to explore the interactions between bacterial and AM fungal communities associated with wheat roots in fields, and their effects on plant benefit from AM fungi. To do this, we characterize the communities by metagenomic approaches and isolate bacterial and AM fungal strains associated with wheat roots. The growth of various wheat cultivars will be measured in the presence or the absence of combinations of isolated bacteria and AM fungi.

Project leader:

  • Benoit Lefebvre, researcher (DR2 INRA)
researcherID logo

Project members:

  • Yi Ding, PhD student (Chinese scholarship)
  • Margot Trinquier, PhD student (fellowship from Toulouse University - Région Occitanie), co-supervised by Christophe Roux (LRSV, Toulouse)
  • Mégane Gaston, assistant IE (WHEATSYM grant)
  • Virginie Gasciolli, Technician

Past members:

  • Marie Cumener, assistant IE (2012-2014)
  • Ariane Girardin, PhD student (2014-2017)
  • Tongming Wang, PhD student (2014-2018), post-doc (2019-2020)
  • Claudia Bartoli, post-doc (2016-2018)
  • Luis Buendia, PhD student (2015-2018, co-supervision with S. Bensmihen)
  • Camille Ribeyre, assistant AI (2018-2019)

Current funding

  • Project MYCOBLE (FSOV, 2021-2023), coordinator: B. Lefebvre
  • Project WHEATSYM (ANR, 2017-2021), coordinator: B. Lefebvre

Past funding

  • Projet Stress'n'Sym (Institut Carnot Plant2Pro, 2017-2020), coordinator: B. Lefebvre
  • Project DIBAM (SPE INRA, 2018-2019), coordinator: B. Lefebvre
  • Projet RHIZOWHEAT (IDEX ATS, 2016-2017), coordinator: C. Masson (LIPM)
  • Projet SPE (INRA, 2014-2015), coordinator: J. Cullimore (LIPM)
  • Projet LCOinNONLEGUMES (ANR young scientist, 2011-2014), coordinator: B. Lefebvre
  • PICS (CNRS, 2011-2012), coordinator: B. Lefebvre

Publications

  • Du D, Zhang C, Xing Y, Lu X, Cai L, Yun H, Zhang Q, Zhang Y, Chen X, Liu M, Sang X, Ling Y, Yang Z, Li Y, Lefebvre B, He G (2020) The CC-NB-LRR OsRLR1 mediates rice disease resistance through interaction with OsWRKY19. Plant Biotechnol J, in press
    PubMed
  • Lefebvre B. 2020. An opportunity to breed rice for improved benefits from the arbuscular mycorrhizal symbiosis? New Phytol, 225:1404-1406
    PubMed
  • Girardin A, Wang T, Ding Y, Keller J, Buendia L, Gaston M, Ribeyre C, Gasciolli V, Auriac MC, Vernié T, Bendahmane A, Ried MK, Parniske M, Vandenbussche M, Schorderet M, Reinhardt D, Delaux PM, Bono JJ and Lefebvre B. 2019. LCO receptors involved in arbuscular mycorrhiza are functional for rhizobia perception in legumes. Current Biol, 29: 4249-4259
    PubMed
  • Buendia L, Ribeyre C, Bensmihen S, Lefebvre B. 2019. Brachypodium distachyon tar2lhypo mutant shows reduced root developmental response to symbiotic signal but increased arbuscular mycorrhiza. Plant Signal Behav 14: e1651608
    PubMed
  • Buendia L., Maillet F., O’Connor D., van de-Kerkhove Q., Danoun S., Gough C., Lefebvre B. and Bensmihen S. 2019. LCOs promote lateral root formation and modify auxin homeostasis in Brachypodium distachyon. New Phytol, 221: 2190-2202
    PubMed
  • Buendia L., Girardin A., Wang T., Cottret L. and Lefebvre B. 2018 LysM Receptor-Like Kinase and LysM Receptor-Like Protein Families: An Update on Phylogeny and Functional Characterization. Front. Plant Sci. 9:1531
    PubMed
  • Gough C., Cottret L., Lefebvre B. and Bono JJ. 2018. Evolutionary History of Plant LysM Receptor Proteins Related to Root Endosymbiosis. Front Plant Sci. 9:923
    PubMed
  • Lefebvre B. 2017. Arbuscular mycorrhiza: A new role for N-acetylglucosamine. Nature Plants 3, 17085
    PubMed
  • Vernié T., Camut S., Camps C., Rembliere C., de Carvalho-Niebel F., Mbengue M., Timmers T., Gasciolli V., Thompson R., Le Signor C., Lefebvre B., Cullimore J. and Hervé C. 2016. PUB1 interacts with the receptor kinase DMI2 and negatively regulates rhizobial and arbuscular mycorrhizal symbioses through its ubiquitination activity in Medicago truncatula. Plant Physiol, 170: 2312-2324
    PubMed
  • Buendia L., Wang T., Girardin A.  and Lefebvre B. 2016. The LysM receptor-like kinase SlLYK10 regulates the arbuscular mycorrhizal symbiosis in tomato. New Phytol 210, 184-195
    PubMed
  • Pietraszewska-Bogiel A., Lefebvre B., Koini M.A., Klaus-Heisen D., Takken F.L.W., Geurts R., Cullimore J.V and Gadella T.W.J. 2013. Interaction of Medicago truncatula Lysin motif receptor-like kinases, NFP and LYK3, produced in Nicotiana benthamianaleaf induces a defence-like response. PlosOne 8(6):e65055
    PubMed
  • Lefebvre B, Klaus-Heisen D, Pietraszewska-Bogiel A, Hervé C, Camut S, Auriac MC, Gasciolli V, Nurisso A, Gadella TW, Cullimore J. 2012. Role of N-glycosylation sites and CxC motifs in trafficking of Medicago truncatula Nod Factor Perception protein to plasma membrane. J Biol Chem 287: 10812-10823
    PubMed
  • Klaus-Heisen, D., Nurisso, A., Pietraszewska-Bogiel, A., Mbengue, M., Camut, S., Timmers, T., Pichereaux, C., Rossignol, M., Gadella, T.W.J., Imberty, A., Lefebvre, B., Cullimore, J.V. 2011. Structure-function similarities between a plant receptor-like kinase and the human interleukin-1 receptor-associated kinase-4. J Biol Chem 286: 11202-11210
    PubMed
  • Mbengue, M., Camut, S., de Carvalho-Niebel, F., Deslandes, L., Froidure, S., Klaus-Heisen, D., Moreau, S., Rivas, S., Timmers, T., Hervé, C., Cullimore, J., Lefebvre, B. 2010. The Medicago truncatula E3 ubiquitin ligase PUB1 interacts with the LYK3 symbiotic receptor and negatively regulates infection and nodulation. Plant Cell 22: 3474-3488
    PubMed
  • Lefebvre, B., Timmers, T., Mbengue, M., Moreau, S., Hervé, C., Tóth, K., Bittencourt-Silvestre, J., Klaus, D., Deslandes, L., Godiard, L., Murray, J.D., Udvardi, M.K., Raffaele, S., Mongrand, S., Cullimore, J., Gamas, P., Niebel, A. and Ott, T. 2010. A remorin protein interacts with symbiotic receptors and regulates bacterial infection. Proc Natl Acad Sci U S A. 107: 2343-2348
    PubMed